Pharmaceuticals

Single crystal X-ray crystallography is a primary means of determining the molecular conformations of proteins and other biomolecules. It is thus critical for such applications as intelligent drug design and the characterization of medically-important effector targets.

The CLS boasts a highly-productive insertion device beamline (08ID-1) as well as a bending magnet beamline (08B1-1) with advanced capabilities that go beyond protein crystallography. Both beamlines are fully-equipped with on-axis visualization and the Stanford Automated Mounting System (SAM) allowing on-site or remote access. The facility has efficient software for sample and experiment tracking, data collection as well as an automated data processing pipeline. A humidity control device is available for controlled crystal dehydration experiments.

Applications

  • Single crystal X-ray crystallography for determining detailed structures of proteins and other molecules.
  • Determining protein structures with bound ligands for rational drug design.
  • Structural proteomics.
  • X-ray absorption spectroscopy (XAS), allowing high-resolution Extended X-ray Absorption Fine Structure (EXAFS) experiments on crystals to characterize metal centres.
  • Rapidly analyzing multi-component mixtures using powder diffraction methods to design pharmaceutical formulations and improve drug delivery systems.

 

 

Synchrotron advantages over conventional research techniques

  • Higher resolutions are usually attainable, allowing for more detailed structures
  • Spectral range of 4-18 keV allows for Single Anomalous Dispersion (SAD) and Multiple Anomalous Dispersion (MAD) experiments as well as characterization of a wide array of metal centres
  • High intensity of light allows for short experiment times on small sample sizes
  • Experimental phasing using sulfur is possible, which is naturally present in protein samples

Discover our areas of expertise

 

Services

  • Unique-in-Canada analytical services:
    • Advanced X-ray imaging
    • Non-destructive testing
    • In-situ investigations
    • Bulk and surface investigations
  • A strong team of accomplished scientists to work with you to develop scientific experiments that meet industry standards
  • A full range of access modes to suit your needs
  • Remote data collection from anywhere in the world
  • On-site data collection, taking advantage of Saskatoon’s central location
  • Mail-in service, where competent staff perform experiments for you
  • Expertise across the scientific spectrum
  • Leading-edge research and development
  • Terms and Conditions

how can we help?

If you’re looking for information on how you can use CLS techniques in your research program, please contact us using this form.

Example queries may include: Feasibility around a potential experiment? A scientific problem we can help you solve? Is your question related to a specific technique? Do you want to know more about how to apply for beamtime?

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