Pharmaceuticals

Single crystal X-ray crystallography is a primary means of determining the molecular conformations of proteins and other biomolecules. It is thus critical for such applications as intelligent drug design and the characterization of medically-important effector targets.

The CLS boasts a highly-productive insertion device beamline (08ID-1) as well as a bending magnet beamline (08B1-1) with advanced capabilities that go beyond protein crystallography. Both beamlines are fully-equipped with on-axis visualization and the Stanford Automated Mounting System (SAM) allowing on-site or remote access. The facility has efficient software for sample and experiment tracking, data collection as well as an automated data processing pipeline. A humidity control device is available for controlled crystal dehydration experiments.

Applications

  • Single crystal X-ray crystallography for determining detailed structures of proteins and other molecules.
  • Determining protein structures with bound ligands for rational drug design.
  • Structural proteomics.
  • X-ray absorption spectroscopy (XAS), allowing high-resolution Extended X-ray Absorption Fine Structure (EXAFS) experiments on crystals to characterize metal centres.
  • Rapidly analyzing multi-component mixtures using powder diffraction methods to design pharmaceutical formulations and improve drug delivery systems.

 

 

Synchrotron advantages over conventional research techniques

  • Higher resolutions are usually attainable, allowing for more detailed structures
  • Spectral range of 4-18 keV allows for Single Anomalous Dispersion (SAD) and Multiple Anomalous Dispersion (MAD) experiments as well as characterization of a wide array of metal centres
  • High intensity of light allows for short experiment times on small sample sizes
  • Experimental phasing using sulfur is possible, which is naturally present in protein samples

Discover our areas of expertise

 

Services

  • Unique-in-Canada analytical services:
    • Advanced X-ray imaging
    • Non-destructive testing
    • In-situ investigations
    • Bulk and surface investigations
  • A strong team of accomplished scientists to work with you to develop scientific experiments that meet industry standards
  • A full range of access modes to suit your needs
  • Remote data collection from anywhere in the world
  • On-site data collection, taking advantage of Saskatoon’s central location
  • Mail-in service, where competent staff perform experiments for you
  • Expertise across the scientific spectrum
  • Leading-edge research and development
  • A trusted reputation

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