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Newsletter========================================
****************************************** 1. CALL FOR PROPOSALS OPEN - DEADLINE WEDNESDAY, SEPTEMBER 8, 2010 Cycle 13 Call for Proposals is open for scheduling of beam time for the January-June 2011 period. ***THE DEADLINE FOR PROPOSALS IS SEPTEMBER 8, 2010*** GENERAL USER PROPOSALS for research time through a competitive peer review process takes place twice per year. Proposals received for operational beamlines are reviewed for safety and technical feasibility, and then scored for scientific merit by an external review committee. General User research should result in the publication of scientific results. You have the option of submitting a NEW proposal or submitting a Beam Time Request against an Active Proposal. Allocation of shifts for January-June will be based on the highest ranked proposals (both new and active). To view evaluation criteria used in reviewing and rating proposals see http://www.lightsource.ca/uso/proposal_writing_tips.php or to compare your score to others on the same beamline, please visit http://www.lightsource.ca/uso/results_of_call.php. View participating Phase I and Phase II beamlines participating in the Call at http://www.lightsource.ca/uso/beamline_status.php In addition to normal mode operations at 250 mA, four shifts are available for Single Bunch Mode and three for Coherent Synchrotron Radiation studies. These shifts must be applied for through the General User program. Reminder: New web developments have been implemented. To add team members to your proposal, you require the email address they have registered with CLS. Please have the list of email addresses handy when completing a NEW proposal form. For more information, please visit our website: http://www.lightsource.ca/uso/call_proposals.php 2. GOVERNOR GENERAL VISITS CLS Her Excellency The Right Honourable Michaëlle Jean, Governor General of Canada, toured the CLS on August 24, 2010. Hosted by CLS Executive Director Josef Hormes, Her Excellency toured the facility, including two CLS beamlines - the Canadian Macromolecular Crystallography Facility (CMCF) and the Biomedical Imaging and Therapy (BMIT) facility- and met with young scientists who are using the CLS in their research. For photos of the visit please go to: http://www.lightsource.ca/governorgeneralvisitsclsaugust24.php 3. ELECTION RESULTS - USERS' ADVISORY COMMITTEE It is my pleasure to inform you of the results of the Canadian Light Source Users' Advisory Committee (UAC) election, held July 12-19, 2010. Congratulations to the following new Regular Members, elected for a three-year term, and to the Student Member, elected for a two-year term, all commencing in September 2010:
On behalf of the UAC, I also would like to thank all nominees in this election. We look forward to working with the new members of our committee. We would like to thank retiring members - Helen Nichol, Farideh Jalilehvand, and Brian Brewer for their valuable contributions to the UAC. Finally, sincere thanks go to the past UAC Chair, Stewart McIntyre, for his dedicated work. If you have any questions or suggestions, the members of the UAC welcome your input at clsuac@lightsource.ca . Information about the UAC can be found at http://www.lightsource.ca/uac Sincerely, 4. RESEARCH HIGHLIGHT: Crystal Structures of Apo and Metal-Bound Forms of the UreE Protein from Helicobacter pylori: Role of Multiple Metal Binding Sites The crystal structure of the urease maturation protein UreE from Helicobacter pylori has been determined in its apo form at 2.1 Å resolution, bound to Cu2+ at 2.7 Å resolution, and bound to Ni2+ at 3.1 Å resolution. Apo UreE forms dimers, while the metal-bound enzymes are arranged as tetramers that consist of a dimer of dimers associated around the metal ion through coordination by His102 residues from each subunit of the tetramer. Comparison of independent subunits from different crystal forms indicates changes in the relative arrangement of the N- and C-terminal domains in response to metal binding. The improved ability of engineered versions of UreE containing hexahistidine sequences at either the N-terminal or C-terminal end to provide Ni2+ for the final metal sink (urease) is eliminated in the H102A version. Therefore, the ability of the improved Ni2+-binding versions to deliver more nickel is likely an effect of an increased local concentration of metal ions that can rapidly replenish transferred ions bound to His102. Rong Shi, Munger, C., Asinas, A., Benoit, S.L., Miller, E., Matte, A., Maier, R.J. and Cygler, M. Crystal Structures of Apo and Metal-Bound Forms of the UreE Protein from Helicobacter pylori: Role of Multiple Metal Binding Sites. Biochemistry, 2010, 49 (33), pp. 7080-7088. DOI: 10.1021/bi100372h. This research was conducted at CMCF 1 by users from the Department of Biochemistry, McGill University, the Department of Microbiology, University of Georgia and the NRC Biotechnology Research Institute. 5. UPCOMING CLS WORKSHOP AT CAPA 2010 The CLS is organizing "Stones, Bones and Photons: Synchrotron applications in physical anthropology, archaeology, palaeontology and preservation studies" in conjunction with the annual meeting of the Canadian Association for Physical Anthropology (CAPA). The half-day workshop takes place at the CLS on Saturday, October 30. The workshop will provide an opportunity for the current and the curious potential user to discuss the application of synchrotron techniques to archaeological, palaeontological and preservation studies. Speakers include:
For more information, please visit: http://www.lightsource.ca/stones&bones/index.php or http://www.medicine.usask.ca/che/capa-conference to register. 6. NEW STAFF & NOW HIRING As the new school year approaches, we would like to thank all our students who contributed their time and expertise to our facility over the summer months. Best wishes in your upcoming studies and careers! We are pleased to welcome the following new employee who recently joined the CLS:
We are currently accepting applications for the following positions:
For the complete postings, please go to: http://www.lightsource.ca/careers/ =============================================== Last modified: 2010-08-31 13:08:12 |
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