Canadian Macromolecular Crystallography Facility (CMCF-ID) 08ID-1

Overview

The scientific goal of the 08ID-1 beamline is to operate a protein crystallography beamline suitable for studying small crystals and crystals with large unit cells. The overall design of the beamline contains white beam slits (WBS), a double crystal monochromator (DCM) with an indirectly cryo-cooled first crystal and sagittally focusing second crystal, and vertically focusing mirror (VFM). The beamline is completed with an innovative and very robust endstation with Mar300CCD X-ray detector. The beamline controls are being developed based on EPICS and are complemented with a Blu-Ice-like user interface.

The beamline is equipped with a Röntek Spectrometer System XFLASH 101A to perform X-ray spectroscopy for MAD and X-ray absorption near edge structure (XANES) on the same crystals, and the X-ray fluorescence (XRF) for metal tracing on protein derivative crystals. There are ongoing efforts to equip the beamline with a cryogenic sample changer to facilitate remote access to the facility.

  • Status

    Accepting Proposals

  • Resolution Δ E

    ≥ 0.001 cm-1

  • Spot size (Horizontal x Vertical)

    130 μm x 20 μm

  • Flux

    5 x 1012 ph/s @ 250 mA and 12 keV

  • Wavelength

    1.9 – 0.7 Å

  • Resolution Δ E / E @ E=12 keV

    10-4

  • Energy Range

    6.5 - 18 keV

  • Source

    20 mm hybrid small gap in-vacuum undulator

Endstations(s):

  • Rontek XFLASH 101A Spectrometer
  • Automounter (under development)
  • Oxford Instruments Cryojet
  • Marmosaic CCD300
  • Huber Model 410 goniometer with single phi axis

Techniques:

  • Single crystal X-ray diffraction
  • Multiwavelength Anomalous Dispersion (MAD)
  • XANES on crystals

Optics:

  • DCM with cryo-cooled 1st crystal sagittally bent 2nd crystal followed by vertically focusing mirror.

Contacts

Beamline Scientist: Pawel Grochulski (306) 657-3538
Science Associate: James Gorin (306) 657-3776
Research Associate: Shaun Labiuk (306) 657-3828
Beamline Team Leader: Miroslaw Cygler 
Beamline Team Members (PDF)
Beamline Telephone: (306) 657-3619

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